Biotechnology Laboratory 
Updated 10/9/03  For this Session there will be BioTech I and Biotech II activities. II activities will be for Bio students who will try some new activities.

NIH Reference Activities are from the Biotechnologist
Center for Image Processing


  • Read Course Outline/sign biosafety agreement 

  • Complete weekly activity sheet 

  • NIH Image Activity - Image Analysis tools

  • Learn how to extract DNA

  • Complete DNA Size Activity during the week


  • Complete session activity report

  • Lab Activity- Colony Transformation

  • NIH Companion exercise- Bacterial Transformation (BTII will have a different activity)

  • Start Human Genome Activity

  • Work on Gel Electrophoresis Exercise during the week (Instructions for scanning gel)



  • Complete session activity report

  • Lab Activity- set up Restriction Mapping

  • NIH Companion Exercise-Restriction Mapping

  • Select Group Projects

  • Annotated Bib #1 due

  • Work on PCR Exercise during the week



  • Complete session activity sheet

  • Learn Protein Assay Procedure and collect data/digital pix

  • Work on PCR lab analysis if needed

  • Work on DNA Sequencing Exercise

  • Get projects set up/schedule for next lab period

  • Annotated Bib #2 due


  • Complete session activity report

  • Complete Project data Collection

  • WWW assignment due( take new pix of set-ups)

  • Work on Write-up (2 groups)

  • Quiz


  • Last day!!!- no late work!!

  • Make sure notebook is cleaned out of your marked papers or points will be deducted


Biotechnology lab continued-Notes


Review Questions: Know terms and techniques for:

1. What is biotechnology and  how is it related to  genetic engineering, cloning, transformations?
2. What are restriction enzymes and how are they used in working with DNA?
3.If you want to extract DNA from bacteria cells outline the basic protocol which must take place.  
What properties of DNA will make the molecule precipitate?
4.  What is DNA and what is it made of?  What are some of the current sources for laboratory study?
5.  What is gel electrophoresis and how is it used in examining DNA and Proteins?
6.  What are the microbiology lab safety concerns you must be aware of in the biotechnology work place? 
7.  If a scientist wants to change the gene in an bacterium  what are the kinds of biological materials needed?  
(hint: vector, host etc.) What is the function of each part of the experiment?  
8.What is the Human Genome Project and what is the current status of this major scientific collaborative project?
9.  How do you do restriction mapping and what does it tell you?
10. How are DNA profiles used in forensic and paternity cases? ( interpret one)
11. What is PCR and how is it an important tool for scientists?

 Keep a list of the key terms you use and protocols.

Reference NIH Exercises, Lab Procedures and Biotechnology Texts in Lab