Creating A Dilution Series
Why is a dilution series important?
The creation of a dilution series in the microbiology laboratory is an essential skill that any researcher in this field of study must know how to do. Such a skill is used to decrease the actual numbers of bacteria colonies growing on a media plate. It is important to know the source of the inoculum, which is the liquid or a solid to make the stock culture bottle.
To create a dilution series:
Autoclave the bottles that will be used.
Pour 99ml Phosphate Buffer into each bottle in the series.
Arrange the bottles in a line and set aside.
Obtain a sterile 1 ml glass pipette.
Draw up the 1ml of your sample and empty it into the first bottle containing the buffer; shake solution.
Using the same method as stated previously, draw up 1ml of the solution made from your sample and the buffer and empty it into the second bottle if needed.
Continue as needed until your solution reaches the desired dilution level.
Once the last buffer solution has been created, use only the bottles within the predicted colony range (usually the last two).
Inoculate the desired plates and incubate by instructions.
CLEAN UP your work area!!!
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