- Place absorbent pad into
petri dish using forceps that have been cleaned with
- Put ampoule of appropriate
medium (M-ColiBlue24) onto pad
- Close dish and mark
appropriately, set aside
- Clean filtration apparatus
with alcohol wipes
- Connect filtration
apparatus using a single glass filter holder, a filter
holder support base, a clamp, and two flasks connected by
tube and connect to Millipore filter (as shown in picture in Millipore
- Load a sterile membrane
onto the center of the filter holder support base, grid
side up, using forceps that have been cleaned with
- Clamp funnel into position.
- Pour water sample into
funnel (100mL of sample is ideal).
- Turn on Millipore filter.
- Lift filter holder off of
funnel and remove filter using sterilized forceps.
- Transfer filter
immediately to prepared petri dish by centering the
filter, grid side up, on the medium soaked. Make sure air
is not trapped under filter, and replace cover tightly.
- Invert petri dish and
incubate for 22 - 24 hours at 35 degrees Celsius
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