Using Photoshop 7.0 in Electron Microscopy


• First pull up your picture by going to File and then Open to choose the picture that needs editing.

• In order to be able to trace your steps, go to Window – History. This will cause the History box to pop up in the right side of the Photoshop screen. Another helpful Window that you should pull up is the Layer window. This can be found under Menu – Windows – Layer. This window is helpful if you are working with many layers on your picture.

• In order to change the size of the image, you must go to the Menu – Image – Image Size. Then a smaller window will pop up. In the Image Size window, the only changes you should make are in the width, height, and resolution. Make sure the box at the bottom next to Constrain Proportions is checked. The resolution should be set at 72 for pictures that will be used online, and 300 for actual prints.

• To change an image from a black and white photo to a color photo, go under Image – Mode – RGB. This will change your image into a color image. After this is done the image will probably need other adjustments. Next you should go to Image – Adjustments – Levels in order to change the contrast on your photo.

• If you are making major changes to you image, you should go to Layer – New – Layer.

• In order to add different effects you can go under Filter in the Menu bar and select any of the actions listed under Filter.

• The Blur tool is effective when you have an area selected and want to blur the background, which brings your image more into focus. In order to use the Blur tool, you must first use the magnetic lasso tool under the selection box in the side toolbar. If a different tool is up and not the magnetic lasso tool, the right click on the box to select the correct tool. The Blur tool will blur the area that you have selected, so if you have selected the image, and want to blur the background, then you need to go to the Menu – Select – Inverse, and then go under the Menu again and go to Filter – Blur – Gaussian Blur. This type of blur has the best results for any type of pictures.

• The Distort tool is another action in Photoshop that can add different effects to the image you have created. Click on the action you would like to use, and see how the image turns out. If you do not like the way your image is looking, then you can simply go to Edit – Step Backward in your menu, and this will save you the trouble of having to start all over with your original image.

• The Sharpen tool is used when you are trying to define your picture. Dr. L likes this used in order to make your pictures sharper and more defined. To pop out the edges and create a greater contrast of the picture's outline, use the following instructions.  Select the parts of the image you wish to pop out with the Wand. Go to the Filter menu and select Sharpen. Under Sharpen, select the Sharpen Edges function. The unsharpen mask tool is used to clear up the whole image.

• The Sketch tool is also an option on the menu that helps create different effects to your picture. The Charcoal item, for example changes your image to look very similar to a charcoal drawing. Try these different sketches, and remember that you can always step backward if you are not satisfied with the outcome from one of the steps on Photoshop.

• If you are trying to magnify your image to work with individual pixels, use the keystrokes (Ctrl +) or (Ctrl -) to zoom in or out as much as you would like.

• In order to enlarge your image or change the size, which is different from your Image size button, and then you must select your image with the selection tool at the top left corner of the toolbox on the left side of your screen. Once the area is selected you can go to Edit - Free Transform or Edit – Transform – and then one of the options under transform. All of these actions can make your image smaller of larger. You just need to try all of them to see which works best for you.

• The Navigator window under Window – Navigator can also help you focus your attention on different parts of your picture. The zoom is the small triangle on the bottom of your navigation window. Slide the triangle to the right to zoom in, and the left to return the image to the normal size.

•When you are finished with your adjustments and are ready to print, or save for the internet, you need to flatten the layers. In order to combine all the layers, go to Layer – Flatten Image and then all components of your picture can be worked on without switching layers.

•After being flattened, save the image as a .PSD file in your group's 6-weeks EM folder. Go to 'Save As' and select the .PSD extension. Then see your instructor to obtain permission and instructions on how to print on the photo printer.