When testing antibiotic resistance, you need to do the following:
To do interpretation, the measurements are compared to a table that tells whether the bacteria is resistant, intermediate, or sensitive to that particular antibiotic. A more in detailed description of the process and the table can be found in Microbiology Applications. Antibiotic resistance is when the bacteria grow "immunities" to the antibiotic for various reasons, enabling them to withstand the antibiotics and live through the treatment. The zone of inhibition, however, is the area around the antibiotic where the bacteria has been "cleared" (see picture). Hence, the bigger the cleared area, or the zone of inhibition, the more effective the antibiotic is. Through the zone of inhibition, it is possible to judge if bacteria has developed an immunity to the antibiotic, or if the antibiotic is potent enough to fight off the bacteria.
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