Mike's Micro Tips
|To avoid contamination, be sure to keep all
lids tightly on bottles, and try not to breathe on any plates of bacteria.
Furthermore, sterilize all bottles with the flame each time they are
opened and closed (remember, ASK BEFORE USING THE FLAME).
||Agar bottle will be HOT following heating
in the microwave. Be sure to wear hot gloves when handling the hot
bottle. (Also, hot agar will easily overflow. Try to prevent
this in the microwave by closely monitoring the heating agar.)
|To get the most use of out your agar, fill
each bacteria plate so that the bottom is three-fourths (ľ) covered with
agar, and "swish" the plate around so the agar will completely cover the
bottom of the plate.
|If your study involves adding a chemical
into the agar, you should pipette the chemical onto each plate before
pouring the plate (500 µl is the amount of chemical generally used when
following this procedure). Then, allow the agar to cool to a
temperature of about 50° to 60°C, pour the plates, the "swish" the plates
in a figure-8 motion to mix the agar and the chemical.
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