Will  and  Mike's  Micro  Tips

To avoid contamination, be sure to keep all lids tightly on bottles, and try not to breathe on any plates of bacteria.  Furthermore, sterilize all bottles with the flame each time they are opened and closed (remember, ASK BEFORE USING THE FLAME).

 

Agar bottle will be HOT following heating in the microwave.  Be sure to wear hot gloves when handling the hot bottle.  (Also, hot agar will easily overflow.  Try to prevent this in the microwave by closely monitoring the heating agar.)
 
To get the most use of out your agar, fill each bacteria plate so that the bottom is three-fourths (ľ) covered with agar, and "swish" the plate around so the agar will completely cover the bottom of the plate.
If your study involves adding a chemical into the agar, you should pipette the chemical onto each plate before pouring the plate (500 µl is the amount of chemical generally used when following this procedure).  Then, allow the agar to cool to a temperature of about 50° to 60°C, pour the plates, the "swish" the plates in a figure-8 motion to mix the agar and the chemical.

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