Think You Have It Figured Out? 

Follow  This  Printable  "Techie  Check  List"  To  Make  Sure  That  Your  NIH Density Slicing  Session  Goes  Smoothly

Picture is digitized into a 72 dpi format and is saved as a TIFF file

Picture is opened in NIH Image

Density slicing is turned on, and LUT is manipulated so that the density slice highlights only the pixels that are bacteria colonies

MEASURE selected to count number of colonies