Mike & Will's Helpful SmartSpec™ Tips
WARNING: Beyond this point you may find EXTREMELY helpful tips concerning how to use the Spectrophotometer to get your project done right!
|Be sure to BLANK the spec — blanking the spec shows the machine what to compare sample readings with, and if it is not blanked to 0.00 cells/ ml, your sample readings will be inaccurate.|
|It's essential that the cuvette is shaken immediately before it is placed into the spec for a reading, whether it be the blank or the actual sample. This can be done by stretching parafilm across the opening and shaking vigorously between the thumb and index finger.|
If the spec yields a negative concentration of bacteria (i.e. -1.6e7 cells/ml) then the bacteria has probably settled toward the bottom of the cuvette, or the sample has been allowed to sit too long, resulting in cell death.
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